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Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
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Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
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Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
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Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
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Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
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Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
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Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient <t>SDS</t> <t>PAGE.</t> Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.
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Image Search Results


Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient SDS PAGE. Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.

Journal: BMC Physiology

Article Title: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta

doi: 10.1186/1472-6793-10-9

Figure Lengend Snippet: Expression of MsSCP-x and MsSCP-2 . (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta . Lane A: Day 1-4 th instar; lane B: Day 3-4 th instar; lane C: Day 1-5 th instar; lane D: Day 4-5 th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4 th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient SDS PAGE. Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.

Article Snippet: Proteins (20 μg) from each sample were resolved on a 4-20% pre-casted gradient SDS PAGE gel (ISC Bioexpress, Kaysville, UT, USA) and transferred onto a Hybond-C extra membrane (Amersham BioScience, Piscataway, NJ, USA) at 18 volts overnight in Tris-glycine transfer buffer (0.303% Tris base, 1.44% glycine, 20% methanol) as described [ ].

Techniques: Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Purification, Recombinant, SDS Page, Marker